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HMVEC were set with 4% paraformaldehyde for 15?min, and also permeabilized with regard to 15?min along with 0��1% Triton X-100. Following pretreatment together with preventing solution (DakoCytomation, Glostrup, Denmark), cells #links# ended up incubated pertaining to 1?h in room temperature with main antibodies versus elastin (1?:?50, bunnie polyclonal; Biologo, Kronshagen, Germany). Proteins exp

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